FastQCFastQC Report
Thu 26 May 2016
SRR1512475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512475_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465602
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA79260.32146307473793423No Hit
GTACATGGGAAGCAGTGGTATCAAC77390.313878720085399No Hit
GTCCTACAGTGGACATTTCTAAATT73930.2998456360758954No Hit
GTATCAACGCAGAGTACTTTTTTTT70860.28739431587093134No Hit
GTCCTAAAGTGTGTATTTCTCATTT68180.2765247594705066No Hit
CTTTAGGACGTGAAATATGGCGAGG62880.2550289949472786No Hit
CCCATGTACTCTGCGTTGATACCAC57370.23268151145237553No Hit
TATCAACGCAGAGTACTTTTTTTTT47170.19131230425672918No Hit
GGTATCAACGCAGAGTACTTTTTTT45770.18563417777889538No Hit
GTCCTACAGTGTGCATTTCTCATTT38970.15805470631513116No Hit
CTGAAGGACCTGGAATATGGCGAGA37180.1507948160327579No Hit
GAGTACATGGGAAGCAGTGGTATCA34790.1411014429741702No Hit
ATTTAGAAATGTCCACTGTAGGACG28390.11514429336121565No Hit
CTGTAGGACCTGGAATATGGCGAGA27780.1126702525387309No Hit
GTCCTTCAGTGTGCATTTCTCATTT27040.10966895711473303No Hit
TTTCTAAATTTTCCACCTTTTTCAG26590.10784384503257217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC250.006022238619.0059054
TGTACGA400.00527151914.2535612
TAGGACC11850.012.7507984
CCTGCGT604.0954194E-412.6659778
GGCGTGC1155.333277E-912.3906298
GGCGAGG17100.011.43941119
AGGACGT40500.011.0987365
GGACGTG39950.010.9858576
TCCGTGC700.001492617110.8565528
TGTAGGA42850.010.7331362
CTACGGT803.7615476E-410.6908224
AGGACCT25600.010.6167955
GACGTGG20700.010.5549817
CTGTAGG42250.010.5134571
GACGTGA21250.010.505317
GTAGGAC41500.010.4869583
AAATGTC9900.010.4590277
TAAGACT3550.010.4398644
TTAGGAC22650.010.4043663
TAGAAAT11650.010.2778714
Produced by FastQC (version 0.11.5)