FastQCFastQC Report
Thu 26 May 2016
SRR1512474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134112
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC64690.30312373483678456No Hit
GTATCAACGCAGAGTACTTTTTTTT61810.2896286605389033No Hit
CCCATGTACTCTGCGTTGATACCAC43030.20162953022146915No Hit
GGTATCAACGCAGAGTACTTTTTTT38070.1783880133751181No Hit
TATCAACGCAGAGTACTTTTTTTTT36510.17107818146376572No Hit
GTCCTACAGTGGACATTTCTAAATT28060.13148325861060714No Hit
GTCCTAAAGTGTGTATTTCTCATTT27910.13078039015759246No Hit
GAGTACATGGGAAGCAGTGGTATCA27810.13031181118891605No Hit
CTGTAGGACGTGGAATATGGCAAGA26620.12473572146166649No Hit
CTTTAGGACGTGAAATATGGCGAGG23570.11044406291703529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG508.8938104E-515.1641185
GGCGAGG8250.011.480419
ATTTAGA5200.011.0577411
TAGGACC6200.010.853354
AGGACGT17700.010.7091235
GTCCTAC15300.010.6913921
GGACGTG16950.010.6797456
CTAGACT1605.675247E-1010.662274
TGCGCCA1252.3121902E-710.61588212
GTATTAG2250.010.5747591
GTATTAC2250.010.5747591
TATACTG2700.010.5306375
GCGCAGA1353.5924677E-710.2810151
GGCTTAA1353.5924677E-710.2810151
GACGTGA10250.010.2635177
CGTGCGC750.002701209410.10917410
TGGCGAG17800.010.05663718
ACGTGAA11950.010.0473258
GCGTGCG856.7588926E-410.0320079
TGTAGGA19300.09.9688742
Produced by FastQC (version 0.11.5)