FastQCFastQC Report
Thu 26 May 2016
SRR1512474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134112
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77340.36239897437435337No Hit
GTACATGGGAAGCAGTGGTATCAAC72750.3408911997121051No Hit
GGTATCAACGCAGAGTACTTTTTTT53550.25092403772622995No Hit
CCCATGTACTCTGCGTTGATACCAC53010.2483937112953772No Hit
TATCAACGCAGAGTACTTTTTTTTT51450.2410838793840248No Hit
GAGTACATGGGAAGCAGTGGTATCA32470.15214759112923784No Hit
CTGTAGGACGTGGAATATGGCAAGA30690.14380688548679732No Hit
GTCCTAAAGTGTGTATTTCTCATTT29990.14052683270606228No Hit
GTCCTACAGTGGACATTTCTAAATT28480.13345129027904815No Hit
CTTTAGGACGTGAAATATGGCGAGG28230.13227984285735706No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25970.12168995816526967No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23540.11030348922643235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG400.00528466914.24808413
TAGACCG500.001496621613.3038235
TTTGCCG655.4576296E-513.15300310
ACCGTGC655.4576296E-513.1530038
TAGGACC6350.011.5229974
TAGGAGT1850.011.3005464
TACACCG600.005864312411.0865215
AGGACCG951.3564137E-511.0031625
GGCGAGG8100.010.785892519
TGGCGAG17800.010.51041518
ACGAAAT1106.053795E-610.36272812
GGACGTG16900.010.1739036
AGGACGT17200.010.1657125
GGACCGT750.002649672810.1326846
ACGAGAC750.00265018810.13244512
TATGGCG19500.010.13078416
CCAATAC1606.148184E-910.09641553
GTAGGAC18300.010.0736973
TTAGGAC10700.010.0353583
CCGTGCA951.64554E-49.9993599
Produced by FastQC (version 0.11.5)