FastQCFastQC Report
Thu 26 May 2016
SRR1512473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837105
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC73490.40003157141263024No Hit
CCCATGTACTCTGCGTTGATACCAC51810.282019808339752No Hit
GTATCAACGCAGAGTACTTTTTTTT51220.27880823360668006No Hit
GGTATCAACGCAGAGTACTTTTTTT33240.1809368544530661No Hit
GAGTACATGGGAAGCAGTGGTATCA31610.1720641988345794No Hit
TATCAACGCAGAGTACTTTTTTTTT31610.1720641988345794No Hit
GTCCTACAGTGGACATTTCTAAATT28580.15557085740880353No Hit
CTGTAGGACGTGGAATATGGCAAGA28020.15252258308588784No Hit
GTCCTAAAGTGTGTATTTCTCATTT26440.1439220948176615No Hit
CTTTAGGACGTGAAATATGGCGAGG23210.1263400839908443No Hit
GTATCAACGCAGAGTACATGGGAAG19450.10587309925126762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5000.012.7000884
AGGACCG550.003123339712.06255
TAGACTG1900.011.9718045
TCCAACG1451.2842065E-911.10173918
GCGCAGA1251.2473174E-711.1011851
TATGCGT600.005980112611.0572924
GTCCTAC14750.011.0205471
GGCGAGG8400.010.71030519
GACGTGG8900.010.5425857
ACTAGAC1002.4620907E-510.4254463
GTAGGAC16950.010.4002843
GTCCTAA9250.010.3939681
TAGGACA4200.010.3803144
TGTAGGA17600.010.2854362
TCCTACA16350.010.2602392
GTATTGG1552.1043888E-810.2315071
GGACGTG18300.010.2027466
AGGACGT19200.010.1687595
AGGACCT11450.09.9329385
GTACTAG907.5490825E-49.9117721
Produced by FastQC (version 0.11.5)