FastQCFastQC Report
Thu 26 May 2016
SRR1512473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837105
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC83770.4559891786261537No Hit
GTATCAACGCAGAGTACTTTTTTTT69770.3797823205532618No Hit
CCCATGTACTCTGCGTTGATACCAC61480.3346569738800994No Hit
TATCAACGCAGAGTACTTTTTTTTT47080.2562727770051249No Hit
GGTATCAACGCAGAGTACTTTTTTT46600.2536599704426257No Hit
GAGTACATGGGAAGCAGTGGTATCA36600.19922650039056017No Hit
CTGTAGGACGTGGAATATGGCAAGA32660.1777797131900463No Hit
GTCCTACAGTGGACATTTCTAAATT30280.16482454731765467No Hit
GTCCTAAAGTGTGTATTTCTCATTT30150.16411691220697783No Hit
CTTTAGGACGTGAAATATGGCGAGG28100.15295805084630437No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22900.12465264641923027No Hit
CATGTACTCTGCGTTGATACCACTG22860.124434912539022No Hit
TATCAACGCAGAGTACATGGGAAGC21920.11931816635412783No Hit
GTATCAACGCAGAGTACATGGGAAG21730.11828393042313858No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20280.11039107726558907No Hit
GCGTTGATACCACTGCTTCCCATGT20070.10924797439449568No Hit
ACGCAGAGTACATGGGAAGCAGTGG19830.10794157111324611No Hit
GGTATCAACGCAGAGTACATGGGAA18660.10157285511715444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG550.003075304412.08749716
GTACTAG657.906934E-411.712921
TAGGACC4800.011.6801464
GCACCGT907.4723994E-611.6097316
GGCGAGG8450.011.23851319
TTAGGAC10950.011.1076333
AGGACGT18550.011.013675
GGACGTG17750.010.9705266
TGTAGGA16450.010.6864022
GACGTGA9850.010.5117277
TCCAACG1201.5303904E-610.28820118
TGGCGAG17300.010.2653318
CTGTAGG17400.010.1730741
GTCCTAC15550.010.0981561
TTTAGGA12450.09.9983342
TATGTCG951.6488823E-49.99717816
TATACAC4300.09.9441983
TAGGACG20450.09.8974614
AGGACCT10900.09.8512075
TATACTG3100.09.8090245
Produced by FastQC (version 0.11.5)