FastQCFastQC Report
Thu 26 May 2016
SRR1512472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068970
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54080.26138610033011594No Hit
GTCCTACAGTGGACATTTCTAAATT49270.23813781736806236No Hit
GTCCTAAAGTGTGTATTTCTCATTT49210.23784781799639435No Hit
CTGTAGGACGTGGAATATGGCAAGA46090.22276785066965687No Hit
CTTTAGGACGTGAAATATGGCGAGG40980.19806957084926316No Hit
GTACATGGGAAGCAGTGGTATCAAC38550.18632459629670803No Hit
GGTATCAACGCAGAGTACTTTTTTT32970.15935465473158142No Hit
TATCAACGCAGAGTACTTTTTTTTT32130.15529466352822902No Hit
GTCCTACAGTGTGCATTTCTCATTT27430.13257804608090015No Hit
CCCATGTACTCTGCGTTGATACCAC25880.12508639564614277No Hit
CTGAAGGACCTGGAATATGGCGAGA22150.10705810137411369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG12750.013.0734519
TAGGACC8000.011.9653374
AGAACCG802.9378556E-511.84686855
GTGTAGG3500.011.0496691
TGGCGAG27950.010.84105518
GTCTAGT1302.1300912E-710.6791691
GGACGTG29200.010.6134966
TTAGGAC17950.010.6126813
AGGACGT29650.010.5802725
CCAACGA909.819021E-510.52313719
TCTACAC3450.010.438983
GTCCTAC25400.010.3067781
TTTAGGA19000.010.225472
GACGTGA15500.010.2112377
CACCTTT8300.010.17274614
ACGTGAA17200.010.1143928
AATGCCG1502.5727786E-810.1002518
TAGAAAT9400.010.0824414
TAATACT2450.010.0577494
TAGAACG856.740231E-410.0349944
Produced by FastQC (version 0.11.5)