FastQCFastQC Report
Thu 26 May 2016
SRR1512472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068970
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70550.3409909278529897No Hit
GTCCTACAGTGGACATTTCTAAATT53640.25925943827121706No Hit
GTCCTAAAGTGTGTATTTCTCATTT53640.25925943827121706No Hit
CTGTAGGACGTGGAATATGGCAAGA53070.25650444424037083No Hit
CTTTAGGACGTGAAATATGGCGAGG48830.23601115530916347No Hit
TATCAACGCAGAGTACTTTTTTTTT46420.22436284721383104No Hit
GGTATCAACGCAGAGTACTTTTTTT45550.22015785632464466No Hit
GTACATGGGAAGCAGTGGTATCAAC43660.21102287611710174No Hit
CCCATGTACTCTGCGTTGATACCAC31750.15345800084099817No Hit
GTCCTACAGTGTGCATTTCTCATTT30480.14731968080735824No Hit
CTGAAGGACCTGGAATATGGCGAGA26520.1281797222772684No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22890.11063476029135269No Hit
CTGTAGGACCTGGAATATGGCGAGA21970.1061881032591096No Hit
ATTTAGAAATGTCCACTGTAGGACG21760.1051731054582715No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21000.10149978008380982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC402.7566083E-416.6293
TAGGACC7600.014.6285184
CGAACTC400.00528840314.24653815
TGGAGCG550.003062402812.0944015
AGGACCT17200.011.6021895
TGCGAGA752.0824048E-411.39530219
GGACCTG16600.011.33103756
GGCGAGG13750.011.25717719
GTCCTAA14800.011.1216551
AATGTCC7850.010.8909158
GCACCGT700.001492080910.8568666
AAATGTC7500.010.63921457
GTCGAGG909.557509E-510.55120619
TGTAGGA28500.010.4358432
AGGACGT29100.010.4170995
GGACGTG28550.010.34824856
TTAGGAC19150.010.1721593
ATTTAGA8350.010.1411831
TACACCG750.002641865810.1362615
GAAATGT8200.010.0790126
Produced by FastQC (version 0.11.5)