FastQCFastQC Report
Thu 26 May 2016
SRR1512471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870390
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT73800.2571079191329401No Hit
CTGTAGGACGTGGAATATGGCAAGA70610.24599444674765447No Hit
GTCCTAAAGTGTGTATTTCTCATTT68810.2397235218907535No Hit
CTTTAGGACGTGAAATATGGCGAGG60180.20965792104905606No Hit
GTATCAACGCAGAGTACTTTTTTTT55750.1942244782067942No Hit
GTCCTACAGTGTGCATTTCTCATTT42500.14806350356571754No Hit
GGTATCAACGCAGAGTACTTTTTTT31960.1113437546814196No Hit
TATCAACGCAGAGTACTTTTTTTTT31160.10855667696724139No Hit
CTGTAGGACCTGGAATATGGCGAGA29180.1016586596246503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14550.013.6133954
GGCGAGG18100.012.08619919
AGGACCT28050.010.9132175
ATTCCGT700.001522180610.8311626
CACCTTT10550.010.7019914
TAGAAAT12450.010.6571684
GTCTAGG2700.010.6473371
TGTAGGA45250.010.6398582
CCACCTT11050.010.55563913
GTCCTAC36900.010.5309241
TGGCGAG44050.010.51171818
GTCCTAA21550.010.488041
GGACCTG27100.010.3165826
GTAGGAC45000.010.256513
AATGTCC11800.010.2549328
GTGTAAG4100.010.15481951
GTCCTAT4400.010.1383341
ACCGGCC750.002758100410.0840188
GTGTAGG6100.010.0755461
TTTAGAA13850.09.9906512
Produced by FastQC (version 0.11.5)