FastQCFastQC Report
Thu 26 May 2016
SRR1512471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870390
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA82910.28884576660314454No Hit
GTCCTACAGTGGACATTTCTAAATT80860.28170387996056284No Hit
GTCCTAAAGTGTGTATTTCTCATTT73720.2568292113615223No Hit
CTTTAGGACGTGAAATATGGCGAGG73570.2563066342901139No Hit
GTATCAACGCAGAGTACTTTTTTTT71740.24993119401893124No Hit
TATCAACGCAGAGTACTTTTTTTTT48280.16820014005065514No Hit
GGTATCAACGCAGAGTACTTTTTTT45280.15774859862248686No Hit
GTCCTACAGTGTGCATTTCTCATTT44980.15670344447967No Hit
CTGAAGGACCTGGAATATGGCGAGA37020.1289720212235968No Hit
CTGTAGGACCTGGAATATGGCGAGA33600.11705726399548493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC508.724044E-515.1987727
GGACCGT851.7200364E-814.5284936
TAGGACC14200.012.9825754
AAGTCGT2600.011.6913637
GGCGAAA1157.11716E-811.56106219
GGCGAGG19800.011.55897619
CAAGTCG2550.011.5482886
TAGTACG752.0694906E-411.4032534
CCACCTT10450.011.27122813
AGTCGTC2650.011.1121178
AGCGCCA600.005882006611.08224610
TGGCGAA1803.6379788E-1211.08031618
CACCTTT10800.010.81725114
AGGACGT45300.010.7825145
TTAGGAC26100.010.6672353
TCCAACG2050.010.65563418
GGACGTG44450.010.6427466
TGGCGAG42700.010.52055318
TGTAGGA48450.010.5122532
AGGACCT28150.010.4987375
Produced by FastQC (version 0.11.5)