FastQCFastQC Report
Thu 26 May 2016
SRR1512470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2877479
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT54720.1901664616839949No Hit
GTATCAACGCAGAGTACTTTTTTTT51900.18036621639984168No Hit
GTCCTAAAGTGTGTATTTCTCATTT48360.16806378083037268No Hit
CTGTAGGACGTGGAATATGGCAAGA47940.1666041698306052No Hit
CTTTAGGACGTGAAATATGGCGAGG40310.14008790333482887No Hit
GTCCTACAGTGTGCATTTCTCATTT36940.1283762626938372No Hit
GGTATCAACGCAGAGTACTTTTTTT31120.1081502245542018No Hit
TATCAACGCAGAGTACTTTTTTTTT30480.10592605541169893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11250.012.4681864
GACCGGG701.1159383E-412.1853677
TACACCG802.9387567E-511.8468845
TCCAACG2400.011.43980118
GTCGTAC700.001047600511.3265351
GCGAAAA600.005955159711.06385413
GTGTAGG5300.011.0326861
ACCGGGT600.006082546411.0324318
GGCGAGG14650.010.85835419
AGGACCT20200.010.7912215
ATAGGAC3900.010.6925723
GGACCTG19150.010.6900346
AGGACGT33750.010.6148075
TGTAGGA36650.010.4989582
GGACGTG33350.010.4863576
GTCCTAT4450.010.46751
GTCCTAA15750.010.4455821
ATCCCGT1553.788955E-910.39849910
TGGCGAG31700.010.36339218
GACGTGG16550.010.2505967
Produced by FastQC (version 0.11.5)