FastQCFastQC Report
Thu 26 May 2016
SRR1512470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2877479
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67580.23485836039116187No Hit
GTCCTACAGTGGACATTTCTAAATT60110.20889813618101125No Hit
CTGTAGGACGTGGAATATGGCAAGA58770.20424128203889585No Hit
GTCCTAAAGTGTGTATTTCTCATTT53690.18658693947027938No Hit
CTTTAGGACGTGAAATATGGCGAGG49030.1703922079014304No Hit
TATCAACGCAGAGTACTTTTTTTTT45370.15767274061774214No Hit
GGTATCAACGCAGAGTACTTTTTTT43470.15106973847593674No Hit
GTCCTACAGTGTGCATTTCTCATTT40660.1413042458346351No Hit
GTACATGGGAAGCAGTGGTATCAAC32100.1115559835536593No Hit
CTGTAGGACCTGGAATATGGCGAGA31380.10905379326834358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13050.012.9605344
TCCAACG1459.822543E-1111.78980318
TGTAGGA36650.011.2256692
GTCTTAC2800.011.2141481
CCGTCCC855.3320407E-511.1752849
TAACACG600.00586867811.0856264
GTATTAG2450.010.8743261
AGAATCG1053.4603727E-610.8593885
GTAGGAC36400.010.702393
AGGACCT22400.010.6897115
AGGACGT32050.010.5840925
CTGTAGG36600.010.5549291
GGACCTG21600.010.5542526
CCAACGA1002.4091318E-510.44653119
GGTATCA19750.010.309961
GACGTGG16600.010.3001127
GGACGTG32200.010.2953136
GGCGAGG13400.010.27643819
TAGAAAT9450.010.2560894
TTAGGAC18400.010.2245853
Produced by FastQC (version 0.11.5)