FastQCFastQC Report
Thu 26 May 2016
SRR1512469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161574
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53170.24597816220957505No Hit
GTACATGGGAAGCAGTGGTATCAAC35470.1640933875037357No Hit
GTCCTAAAGTGTGTATTTCTCATTT34780.16090126916774536No Hit
GTCCTACAGTGGACATTTCTAAATT33720.1559974352023109No Hit
GGTATCAACGCAGAGTACTTTTTTT32790.15169501483641087No Hit
TATCAACGCAGAGTACTTTTTTTTT32540.1505384502219216No Hit
CTGTAGGACGTGGAATATGGCAAGA29860.13814007755459679No Hit
CTTTAGGACGTGAAATATGGCGAGG28070.1298590749148537No Hit
CCCATGTACTCTGCGTTGATACCAC23700.10964232545358152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7250.013.7241344
GTGCGTT701.1135097E-412.1879211
GTATAAT3400.011.6567321
GGACCTG13600.011.2878126
GGCGAGG8500.011.25470919
AGGACCT13850.011.0156415
GGACGTG19800.010.8641156
TATACCG700.001523362410.8299295
GACGTGG9600.010.7594217
GTAGGAC21150.010.753123
TGGCGAG21000.010.73319318
TGTAGGA22650.010.7103932
GTCCTAA10000.010.7008791
AGGACGT20300.010.5965265
GTGTAGG2900.010.5915461
GTCCTAC19750.010.1339791
GTCTTAG2550.010.10251
GTTCTAA3750.010.0403311
CTAGGAC2750.09.9930713
ATGGCGA22550.09.93104717
Produced by FastQC (version 0.11.5)