FastQCFastQC Report
Thu 26 May 2016
SRR1512469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512469_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161574
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70590.32656758454718643No Hit
TATCAACGCAGAGTACTTTTTTTTT48620.2249286862258706No Hit
GGTATCAACGCAGAGTACTTTTTTT45880.2122527380510683No Hit
GTACATGGGAAGCAGTGGTATCAAC38820.17959135333789172No Hit
GTCCTAAAGTGTGTATTTCTCATTT36680.1696911602378637No Hit
CTGTAGGACGTGGAATATGGCAAGA35630.16483358885700883No Hit
GTCCTACAGTGGACATTTCTAAATT35460.16404712491915613No Hit
CTTTAGGACGTGAAATATGGCGAGG32840.15192632775930873No Hit
CCCATGTACTCTGCGTTGATACCAC28570.1321722041438322No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23080.10677404520964816No Hit
GTCCTACAGTGTGCATTTCTCATTT22290.1031193010278621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC853.948444E-612.29324910
TAGGACC7800.012.0607474
TAATACT3200.011.58105854
ACGAGAC600.00588203211.08203812
GGCGAGG10450.010.72353819
AGGACGT21100.010.7188515
GTATTAG1605.311449E-1010.7028421
GACGTGA11550.010.5271517
CCGTGCA1002.4014334E-510.4492639
AATCCCG1553.6852725E-910.41572719
GGACGTG21350.010.366716
TTAGGAC13450.010.3146423
GTCCTAC19150.010.283671
GTAGGAC22900.010.0831073
GCGTGCG856.599449E-410.0581139
GTCCTAA11550.09.9666681
TGGCGAG21650.09.95773218
TAGGACG22650.09.9429164
CCCTATA1252.5631816E-69.8822762
GTCTTAG2800.09.8534111
Produced by FastQC (version 0.11.5)