FastQCFastQC Report
Thu 26 May 2016
SRR1512468_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512468_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717837
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33060.4605502363349897No Hit
GGTATCAACGCAGAGTACTTTTTTT20370.28376915650767515No Hit
TATCAACGCAGAGTACTTTTTTTTT19030.2651019660452164No Hit
GTACATGGGAAGCAGTGGTATCAAC17120.23849425426663712No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10720.14933752369966996No Hit
CCCATGTACTCTGCGTTGATACCAC10540.146829990652474No Hit
GTCCTACAGTGGACATTTCTAAATT10120.1409790802090168No Hit
GTCCTAAAGTGTGTATTTCTCATTT9840.13707847324671202No Hit
CTGTAGGACGTGGAATATGGCAAGA9610.13387440324196162No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9260.1289986445390806No Hit
CTTTAGGACGTGAAATATGGCGAGG8310.1157644423455464No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8080.11256037234079602No Hit
GAGTACATGGGAAGCAGTGGTATCA7350.10239093276050133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA400.00394998314.8843751
CGTGCGC400.00534887114.22032210
TCAATAC551.9998591E-413.783613
CTTATAC551.9998591E-413.783613
GGAGTGT903.749119E-813.687896
GTCCTAG602.828751E-413.2305561
GGCGAGG2950.012.52088719
CTAGAAC854.046633E-612.2633593
TGTATAC854.046633E-612.2633593
GATATAG655.557613E-412.2128211
CTGTGCG701.1136217E-412.1854339
GGTGACC701.1401019E-412.1547968
TGTCCAC2500.012.13467510
TATACTG1251.4606485E-912.1295785
CTAGGGT550.003123704612.0606594
AAATGTC2150.011.9003847
ATGTCCA2400.011.846959
GACGTGA3550.011.745197
TTAGGGT658.184279E-411.6630554
ATTACAC658.184279E-411.6630553