FastQCFastQC Report
Thu 26 May 2016
SRR1512467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421573
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47140.3316044972716843No Hit
GGTATCAACGCAGAGTACTTTTTTT30650.21560623337668908No Hit
TATCAACGCAGAGTACTTTTTTTTT28960.20371799408120442No Hit
GTACATGGGAAGCAGTGGTATCAAC26410.18578011822115362No Hit
CCCATGTACTCTGCGTTGATACCAC18520.13027821997181993No Hit
GTCCTACAGTGGACATTTCTAAATT17680.12436927262968556No Hit
GTCCTAAAGTGTGTATTTCTCATTT15570.10952655966313372No Hit
CTGTAGGACGTGGAATATGGCAAGA15330.10783828899395248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTCG602.5971238E-514.23292714
GCGCTAC450.00868395413.2177091
CGCATTT550.00313715412.05508319
CTAATAC1451.0186341E-1011.7639813
TAGGACC3350.011.3152714
AATCCCG1053.590265E-610.82497319
TAGAAAT3800.010.7234534
GTCTTAG1302.1370943E-710.6758421
TTAGGAC5250.010.2888153
GTCCTAA4750.010.2263331
GTGTAGC3300.010.2136851
GCCTCGA1502.4896508E-810.12083316
CACTGCG856.7436934E-410.0339847
AGTCGTC856.896431E-410.0095638
GAAGTGC951.6851E-49.9753056
ACTGATC1054.3308726E-59.9036428
TGTCCAC4400.09.69362910
GTTTAGA2154.5474735E-119.682741
GTGTAGG1957.767085E-109.6590961
GTATAGG1156.973958E-59.4822691
Produced by FastQC (version 0.11.5)