FastQCFastQC Report
Thu 26 May 2016
SRR1512467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512467_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421573
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55430.38992017996965334No Hit
GGTATCAACGCAGAGTACTTTTTTT39690.27919776191584955No Hit
TATCAACGCAGAGTACTTTTTTTTT38130.2682240025661714No Hit
GTACATGGGAAGCAGTGGTATCAAC30300.21314417198413307No Hit
CCCATGTACTCTGCGTTGATACCAC22810.16045605818343484No Hit
CTGTAGGACGTGGAATATGGCAAGA18800.13224786908586475No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18630.1310520106951947No Hit
GTCCTACAGTGGACATTTCTAAATT18030.12683133402224156No Hit
GTCCTAAAGTGTGTATTTCTCATTT17230.12120376512497072No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16530.11627964233985873No Hit
CTTTAGGACGTGAAATATGGCGAGG16180.11381758094730274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATG500.001499583813.2998288
GTCCTAA4550.012.7500071
TAGGACC4100.012.5164384
CGGACTG550.003060443612.0950095
GTAGGAC10650.011.3312843
TGTAGGA11000.011.143512
ACATTCG803.7706972E-410.6873628
TGGCGAG9900.010.55170618
AAGTCGT1002.3983193E-510.4498647
CAAGTCG1002.399251E-510.4494966
GGCGAGG4550.010.43538619
CTGTAGG10700.010.399061
GTCCTAC8800.010.374821
TAATACC1106.0184793E-610.367154
GGACGTG9700.010.28301056
TATACTG1856.002665E-1110.2737535
GACGTGG5050.010.1582847
AGGACGT9650.010.1433345
CCAATAC856.5780693E-410.06117253
GTCCTAT951.6258049E-410.0107911
Produced by FastQC (version 0.11.5)