FastQCFastQC Report
Thu 26 May 2016
SRR1512466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977310
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT52740.1771397671052057No Hit
GTATCAACGCAGAGTACTTTTTTTT49630.16669409634871746No Hit
GTACATGGGAAGCAGTGGTATCAAC47190.15849877909925403No Hit
CTGTAGGACGTGGAATATGGCAAGA45040.15127749545730879No Hit
GTCCTAAAGTGTGTATTTCTCATTT44470.14936301560804888No Hit
CTTTAGGACGTGAAATATGGCGAGG34070.11443215520049978No Hit
CCCATGTACTCTGCGTTGATACCAC33010.1108718944281919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10600.013.5899074
TCCAACG2200.011.19160718
AGGACGT26600.010.9735665
TGTAGGA31000.010.8527062
GTCCTAC25350.010.8218921
GGACGTG26200.010.7070336
GTAGGAC30250.010.6520273
GTCCTAA12550.010.5745631
AATGTCC7700.010.3164528
AGGACCT21100.010.2856495
GGCGAGG12900.010.27871119
CTGTAGG28450.010.1996851
TTAGGAC15850.010.1647893
TAGGACG29300.010.1240764
TCTATAC3750.010.1089833
GACGTGG14850.010.0834557
ACTGTTC7050.010.060378
TCCTACA30300.010.0400032
CGTGCGC951.680225E-49.97910
GGACCTG21600.09.9159296
Produced by FastQC (version 0.11.5)