FastQCFastQC Report
Thu 26 May 2016
SRR1512466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977310
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66920.22476665177626784No Hit
CTGTAGGACGTGGAATATGGCAAGA54130.18180841094813774No Hit
GTCCTACAGTGGACATTTCTAAATT53730.18046491631707817No Hit
GTACATGGGAAGCAGTGGTATCAAC52380.1759306219372521No Hit
GTCCTAAAGTGTGTATTTCTCATTT45950.15433394574296933No Hit
TATCAACGCAGAGTACTTTTTTTTT44540.1495981271684843No Hit
GGTATCAACGCAGAGTACTTTTTTT42980.14435849810735193No Hit
CTTTAGGACGTGAAATATGGCGAGG42880.14402262444958702No Hit
CCCATGTACTCTGCGTTGATACCAC40930.13747308812317158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10950.012.40924554
TCCAACG2050.011.117969518
GTCCTAC28300.010.4537491
GATATAC6100.010.4482381
AGGACGT27200.010.2012185
TGTAGGA30650.010.137392
TAAAGCG750.002643004510.1360045
CAGCGCG750.00264964410.1329399
CTAGGAC3950.010.1032493
GTAGGAC29500.010.01722053
TTAGGAC16600.010.0170273
TAGGACG28850.09.9797644
GGACCTG18850.09.9782246
GGACGTG27300.09.9518276
TCCTACA31950.09.9033532
TGGCGAG27850.09.8887418
CTGTAGG30750.09.8683291
GGCGAGG12500.09.80032519
TTTAGGA18600.09.7572922
ATAGGAC4800.09.69982053
Produced by FastQC (version 0.11.5)