FastQCFastQC Report
Thu 26 May 2016
SRR1512465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546091
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44410.2872405311200958No Hit
GTACATGGGAAGCAGTGGTATCAAC44280.2863997009231669No Hit
CCCATGTACTCTGCGTTGATACCAC31410.20315751142720578No Hit
GGTATCAACGCAGAGTACTTTTTTT27250.17625094512548098No Hit
GTCCTACAGTGGACATTTCTAAATT25350.1639618884011355No Hit
TATCAACGCAGAGTACTTTTTTTTT25150.16266830348278336No Hit
GTCCTAAAGTGTGTATTTCTCATTT23840.15419532226757676No Hit
CTGTAGGACGTGGAATATGGCAAGA23680.15316045433289502No Hit
CTTTAGGACGTGAAATATGGCGAGG21200.1371200013453283No Hit
GAGTACATGGGAAGCAGTGGTATCA20280.1311695107209084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC509.080264E-515.1267638
CCAACGA802.0688822E-613.0227919
CACCGCT550.003122421712.0627747
TAGGACC4300.011.4616384
CCTACAC2200.011.2011473
ACCCGGT700.001519002610.83328612
GCATTAG650.007745852710.6787581
TCCAACG1252.3446046E-710.60455418
TTACACC909.741358E-510.53099254
GTGTAGG2850.010.4378831
GCGCCAC1002.4458119E-510.43144813
GTCCTAG2001.0913936E-1110.4117891
TGCGCCA1106.204631E-610.34086512
GCCCTAT1251.4837715E-610.3126291
GGCGAGG7900.010.31033219
GGACTGT1502.5331246E-810.1097536
CTAACCT856.7355647E-410.0354174
AGGACGT16700.09.9319245
GTATAGG1405.891834E-79.9159891
TGTAGGA15700.09.8401052
Produced by FastQC (version 0.11.5)