FastQCFastQC Report
Thu 26 May 2016
SRR1512465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546091
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53290.34467570149493143No Hit
GTACATGGGAAGCAGTGGTATCAAC52160.33736694670624173No Hit
CCCATGTACTCTGCGTTGATACCAC37730.2440347948471338No Hit
GGTATCAACGCAGAGTACTTTTTTT37670.2436467193716282No Hit
TATCAACGCAGAGTACTTTTTTTTT35950.23252188907379967No Hit
CTGTAGGACGTGGAATATGGCAAGA28910.18698769994780384No Hit
GTCCTACAGTGGACATTTCTAAATT27650.17883811496218527No Hit
GTCCTAAAGTGTGTATTTCTCATTT27020.17476332246937598No Hit
CTTTAGGACGTGAAATATGGCGAGG25170.16279766197461856No Hit
GAGTACATGGGAAGCAGTGGTATCA24320.1572999260716219No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17300.1118950954374613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4250.012.2983274
ACTGTTC3500.011.6705158
AATCCCG1701.2732926E-1111.17125819
TCTACAC2650.011.1166533
TAGGCCC600.00586067911.0871294
AGGACGT16750.010.9503715
TAGGACT2350.010.9186324
GGACGTG16600.010.8729936
ATTACAC1053.4527166E-610.860513
GATATAC3650.010.687341
ACTAGAC1355.659058E-810.5588283
TTAGGAC10150.010.3923843
GACGTGA8600.010.272457
ATACACT5050.010.1618954
GTAGGAC16650.010.1593053
GACGTGG9000.010.132547
GTATAAA2350.010.1216381
TGTAGGA17350.010.0229552
GGCGAGG7400.010.00884219
TAGGACG18500.09.9655734
Produced by FastQC (version 0.11.5)