FastQCFastQC Report
Thu 26 May 2016
SRR1512464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153761
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT56780.2636318514449839No Hit
GTATCAACGCAGAGTACTTTTTTTT53420.24803123466345614No Hit
GTCCTAAAGTGTGTATTTCTCATTT50000.2321520354394011No Hit
CTGTAGGACGTGGAATATGGCAAGA49320.22899476775742528No Hit
CTTTAGGACGTGAAATATGGCGAGG40540.1882288703342664No Hit
TATCAACGCAGAGTACTTTTTTTTT31900.14811299861033791No Hit
GGTATCAACGCAGAGTACTTTTTTT31170.14472357889292267No Hit
GTACATGGGAAGCAGTGGTATCAAC27260.12656928972156148No Hit
GTCCTACAGTGTGCATTTCTCATTT26850.12466564303095841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC604.1684133E-412.63933410
TAGGACC10450.011.9713124
GTGCGCC907.643781E-611.58632711
GTCCTAC26750.011.4152331
GTGTAGG3400.011.3721751
TGTAGGA32500.011.139462
GGCGAGG12700.011.0361619
GACGTGG15650.011.0215127
TCCTACA31500.010.891362
CCTACAG31400.010.8354993
GTAGGAC32850.010.6457213
AGGACCT19500.010.5951225
CTGTAGG30750.010.4784261
AATGTCC6600.010.4590318
AGGACGT32950.010.4408375
GGACGTG32100.010.392546
TAGGACA8800.010.2311634
GTATTAG1852.9831426E-1010.1821551
ATTATAC2700.010.179313
TGTCGAG1401.0185613E-710.14406118
Produced by FastQC (version 0.11.5)