FastQCFastQC Report
Thu 26 May 2016
SRR1512464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153761
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67190.31196590522346723No Hit
CTGTAGGACGTGGAATATGGCAAGA61280.28452553463453No Hit
GTCCTACAGTGGACATTTCTAAATT59620.2768180870579419No Hit
GTCCTAAAGTGTGTATTTCTCATTT52290.2427845986625257No Hit
CTTTAGGACGTGAAATATGGCGAGG49040.22769471635896463No Hit
TATCAACGCAGAGTACTTTTTTTTT44690.20749748927573675No Hit
GGTATCAACGCAGAGTACTTTTTTT44250.20545455136386997No Hit
GTCCTACAGTGTGCATTTCTCATTT30500.14161274161803467No Hit
GTACATGGGAAGCAGTGGTATCAAC30240.1404055510337498No Hit
CTGAAGGACCTGGAATATGGCGAGA27600.12814792356254942No Hit
CTGTAGGACCTGGAATATGGCGAGA23350.10841500055020033No Hit
CCCATGTACTCTGCGTTGATACCAC22850.1060934801958063No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22710.105443454496576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCA402.7633077E-416.623769
GGATCGT551.9615583E-413.8174726
GGCCGCA500.001500454813.2993177
TAGGACC9250.012.6364794
GGCGAGG12350.011.91748119
TACATCG752.0683592E-411.4036535
TGTAGGA30500.011.1536152
ACCGTTC600.005880166311.0825078
AGGACCT19400.010.7276625
GGACCTG19400.010.6747096
ACGAAAT1701.382432E-1010.61660912
CTGTAGG30150.010.6022811
AATCCCG1451.4546458E-810.47783919
AGGACGT29500.010.4694555
TGGCGAG29700.010.42368518
TGTACTG3400.010.3415475
TGGACTG3050.010.2819815
GTAGGAC31200.010.2028783
GGACGTG29450.010.16076856
TAGGACT3750.010.13657954
Produced by FastQC (version 0.11.5)