FastQCFastQC Report
Thu 26 May 2016
SRR1512463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282982
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC60450.26478526768936417No Hit
GTATCAACGCAGAGTACTTTTTTTT53240.23320376595172454No Hit
CCCATGTACTCTGCGTTGATACCAC40610.1778813849605472No Hit
GTCCTACAGTGGACATTTCTAAATT32370.1417882401175305No Hit
GGTATCAACGCAGAGTACTTTTTTT32240.14121880943432755No Hit
CTGTAGGACGTGGAATATGGCAAGA31570.13828405129782012No Hit
TATCAACGCAGAGTACTTTTTTTTT31550.13819644657732738No Hit
GTCCTAAAGTGTGTATTTCTCATTT31080.13613773564574752No Hit
GAGTACATGGGAAGCAGTGGTATCA25560.11195883278974604No Hit
CTTTAGGACGTGAAATATGGCGAGG24820.10871745813151397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5700.011.6373624
GTATAGG2800.011.3244831
ATCCCGT855.4437354E-511.15232310
GTACTAG1251.2529017E-711.0979931
GATATAC3600.011.0099131
ATAGACG700.001523487210.8298713
GTCCTAC17700.010.7486611
GTCCTAA9500.010.534751
TCCTACA20050.010.4925132
AGGACGT18300.010.4082165
GGACGTG18450.010.1695146
GTAGGAC19300.010.1635273
AATCCCG1502.5625923E-810.1029919
TAGGACA6300.010.0777974
TCGAACT1054.2440173E-59.92257919
GTGTAGG3400.09.9089221
TCCAACG1356.7293877E-79.82083918
CCTACAG20950.09.7701463
GACGTGG9900.09.7632937
CCTATAC1651.065564E-89.7632933
Produced by FastQC (version 0.11.5)