FastQCFastQC Report
Thu 26 May 2016
SRR1512463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282982
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68970.3021048786192795No Hit
GTACATGGGAAGCAGTGGTATCAAC67680.2964543741474966No Hit
CCCATGTACTCTGCGTTGATACCAC50190.2198440460765788No Hit
GGTATCAACGCAGAGTACTTTTTTT44570.19522711961811348No Hit
TATCAACGCAGAGTACTTTTTTTTT44260.19386924645047574No Hit
CTGTAGGACGTGGAATATGGCAAGA37350.1636018155202275No Hit
GTCCTACAGTGGACATTTCTAAATT35350.15484134347095158No Hit
GTCCTAAAGTGTGTATTTCTCATTT32230.14117500707408118No Hit
GAGTACATGGGAAGCAGTGGTATCA30350.13294016334776182No Hit
CTTTAGGACGTGAAATATGGCGAGG29630.12978639341002252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7250.012.5820244
TAGACCG550.003064249412.0935175
TCCAACG1103.8346116E-812.08689518
TAGGACT3850.011.3530994
GACGCTC700.001492727810.8563918
ATAGGAC2900.010.8122033
TGTAGGA21250.010.73125652
GGCGAGG8850.010.73066519
AGGACCT14950.010.5507715
GTAGGAC21300.010.527633
CTGTAGG21350.010.429051
TGGCGAG19100.010.39183718
GCGCCAC1106.0593957E-610.36201213
GTATTAA2500.010.2766271
AGGACGT20750.010.12025455
GTACCGT856.599662E-410.0581276
TCGAACT1701.5697879E-910.055263519
AGGACTG4450.010.0358725
GGACGTG20450.09.9870846
GTCCTAC18300.09.9313681
Produced by FastQC (version 0.11.5)