FastQCFastQC Report
Thu 26 May 2016
SRR1512462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306296
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60250.26124140179751426No Hit
GTCCTACAGTGGACATTTCTAAATT55280.23969169612226704No Hit
GTCCTAAAGTGTGTATTTCTCATTT52820.2290252422065511No Hit
CTGTAGGACGTGGAATATGGCAAGA48010.20816928963151302No Hit
CTTTAGGACGTGAAATATGGCGAGG44810.1942942276273297No Hit
GTACATGGGAAGCAGTGGTATCAAC43280.18766021360657956No Hit
GGTATCAACGCAGAGTACTTTTTTT36740.15930305563552988No Hit
TATCAACGCAGAGTACTTTTTTTTT35670.1546635817778811No Hit
GTCCTACAGTGTGCATTTCTCATTT28340.12288101787454862No Hit
CCCATGTACTCTGCGTTGATACCAC28010.12145015210536722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8600.012.6729124
GGCGAGG14550.012.04235519
GTGTAGG4200.011.3252571
AATGTCC8450.010.9656688
AGGACCT18800.010.8886225
AGGACGT33350.010.7985335
GGACGTG32600.010.7853276
TAGAAAT9600.010.46433454
GTCCTAA15750.010.4444041
TCCAACG2001.8189894E-1210.416903518
GGACCTG19150.010.3926796
GATATAC5000.010.3059841
AAATGTC8650.010.298857
GAAATGT9300.010.2923716
GACGTGA18350.010.2776557
TGTAGGA30900.010.2747862
ACGTGAA20000.010.2114988
GACGTGG16250.010.2061437
GTCCTAC29700.010.1765261
TGGCGAG31800.010.06550418
Produced by FastQC (version 0.11.5)