FastQCFastQC Report
Thu 26 May 2016
SRR1512462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306296
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75550.3275815420050158No Hit
GTCCTACAGTGGACATTTCTAAATT59570.2582929511216253No Hit
CTGTAGGACGTGGAATATGGCAAGA58210.2523960497698474No Hit
GTCCTAAAGTGTGTATTTCTCATTT56770.2461522718679649No Hit
CTTTAGGACGTGAAATATGGCGAGG52730.22863500608768347No Hit
GGTATCAACGCAGAGTACTTTTTTT51510.22334513869858857No Hit
TATCAACGCAGAGTACTTTTTTTTT48690.211117740307402No Hit
GTACATGGGAAGCAGTGGTATCAAC47100.2042235688740734No Hit
CCCATGTACTCTGCGTTGATACCAC34260.1485498825822878No Hit
GTCCTACAGTGTGCATTTCTCATTT30840.13372091006531686No Hit
CTGAAGGACCTGGAATATGGCGAGA28420.12322789442465322No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24930.10809540492634076No Hit
ATTTAGAAATGTCCACTGTAGGACG24420.10588406691942405No Hit
CTGTAGGACCTGGAATATGGCGAGA23300.10102779521795988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCC456.768067E-414.7766311
TCCAACG1352.7284841E-1112.66238918
TAGGACC10250.011.95957854
GGCGAGG13250.011.32422919
CACCTTT8250.010.70686614
TAAGACG803.762031E-410.6906124
TAAGACT3350.010.2119284
TAGAAAT9500.010.202974
AGGACCT20400.010.1551375
CCACCTT8300.010.07063413
CTAGGAC3400.010.060883
TGTAGGA34000.09.9770382
AGGACGT32250.09.9597035
AAGACGT1054.0924584E-59.9554945
TGGCGAG33900.09.94501818
GGACCTG19700.09.9336716
GACGTGG16300.09.9074227
CTGTAGG33650.09.866041
GGACGTG31600.09.8604226
GTAGGAC34000.09.8373043
Produced by FastQC (version 0.11.5)