FastQCFastQC Report
Thu 26 May 2016
SRR1512461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290575
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC63180.4895492319315034No Hit
GTATCAACGCAGAGTACTTTTTTTT47370.3670456966855859No Hit
CCCATGTACTCTGCGTTGATACCAC44190.3424055169207524No Hit
GGTATCAACGCAGAGTACTTTTTTT31070.2407454041803072No Hit
TATCAACGCAGAGTACTTTTTTTTT27770.21517540631114038No Hit
GAGTACATGGGAAGCAGTGGTATCA27100.20998392189527926No Hit
GTCCTAAAGTGTGTATTTCTCATTT18300.1417972609108343No Hit
GTCCTACAGTGGACATTTCTAAATT18280.14164229122677877No Hit
GTATCAACGCAGAGTACATGGGAAG17480.1354435038645565No Hit
CTGTAGGACGTGGAATATGGCAAGA17080.13234411018344536No Hit
GCGTTGATACCACTGCTTCCCATGT15970.12374329271836197No Hit
ACGCAGAGTACATGGGAAGCAGTGG15550.12048892935319529No Hit
CATGTACTCTGCGTTGATACCACTG15450.11971408093291751No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15110.11707959630397304No Hit
GGTATCAACGCAGAGTACATGGGAA15010.11630474788369524No Hit
TATCAACGCAGAGTACATGGGAAGC14860.11514247525327857No Hit
CTTTAGGACGTGAAATATGGCGAGG14580.11297289967650077No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14130.10948608178525075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG307.8424776E-418.95621110
TACACCG400.005365395514.2149435
TAGGACC3750.013.1409244
GATATAC1800.012.1202921
GGACGGG550.003125059712.0611636
GTCTTAT1207.032031E-811.5693681
GTACTAA802.533703E-411.1561781
CAAGACT1408.6674845E-910.8304334
ATGTAGG1957.2759576E-1210.6794181
CTACACC1605.675247E-1010.6612074
TGTAGGA12650.010.5628812
GTCCTAC10350.010.5393851
GCTATAT854.438932E-410.4999311
GTAGGAC12550.010.4960263
GTACAAG2200.010.3673571
GGACCAT1201.5713176E-610.2663486
GGCGAGG5100.010.21392419
ACTACAC1401.00320904E-710.153533
TCTAGTA856.7422184E-410.0340772
AGGACGT11900.09.9544415
Produced by FastQC (version 0.11.5)