FastQCFastQC Report
Thu 26 May 2016
SRR1512461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290575
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC74040.5736977703736706No Hit
GTATCAACGCAGAGTACTTTTTTTT59370.4600275071189199No Hit
CCCATGTACTCTGCGTTGATACCAC52570.40733781454003065No Hit
GGTATCAACGCAGAGTACTTTTTTT42640.33039536640644673No Hit
TATCAACGCAGAGTACTTTTTTTTT40890.3168355190515855No Hit
GAGTACATGGGAAGCAGTGGTATCA32790.2540727970090851No Hit
CTGTAGGACGTGGAATATGGCAAGA21620.16752222846405673No Hit
GTCCTACAGTGGACATTTCTAAATT21270.1648102589930845No Hit
GTCCTAAAGTGTGTATTTCTCATTT20760.1608585320496678No Hit
CATGTACTCTGCGTTGATACCACTG20560.1593088352091122No Hit
ACGCAGAGTACATGGGAAGCAGTGG19630.15210274490052883No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19440.15063053290200107No Hit
TATCAACGCAGAGTACATGGGAAGC19310.14962322995563992No Hit
GTATCAACGCAGAGTACATGGGAAG19020.14737616953683436No Hit
CTTTAGGACGTGAAATATGGCGAGG18460.14303701838327879No Hit
GCGTTGATACCACTGCTTCCCATGT17600.13637332196888985No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17450.13521104933847317No Hit
GGTATCAACGCAGAGTACATGGGAA17410.13490110997036206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3450.013.2194414
GTATTAG1353.6379788E-1011.9778191
GTAGGAC10750.011.7548553
TGTAGGA11000.011.7463662
GACGTGG5600.011.5352687
GTGTAGG1553.0559022E-1011.04595851
AGGACGT11550.010.8588275
CTGTAGG11500.010.7524661
GGACGTG11550.010.692646
CCTACAC2050.010.6597773
GGCGAGG5200.010.59245919
AACCATC1355.6845238E-810.5551467
GTCCTAA5900.010.4790991
GTCTAGT1002.3648881E-510.4629761
TTAGACT1002.3934068E-510.451624
GTGTAGA2053.6379788E-1210.2077831
GCTACAC1409.6610165E-810.1797563
TTATATC750.002644498410.1345113
GGGTTAT750.002647902110.132947
ATTAGAC856.5842853E-410.0599933
Produced by FastQC (version 0.11.5)