FastQCFastQC Report
Thu 26 May 2016
SRR1512460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817457
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC64540.35511156522547715No Hit
GTATCAACGCAGAGTACTTTTTTTT50320.2768703743747445No Hit
CCCATGTACTCTGCGTTGATACCAC45570.25073495549000613No Hit
GTCCTACAGTGGACATTTCTAAATT41610.2289462694303084No Hit
GTCCTAAAGTGTGTATTTCTCATTT40200.22118817666662816No Hit
CTGTAGGACGTGGAATATGGCAAGA39530.21750170705551766No Hit
CTTTAGGACGTGAAATATGGCGAGG33200.1826728225206979No Hit
TATCAACGCAGAGTACTTTTTTTTT29870.16435051833413392No Hit
GGTATCAACGCAGAGTACTTTTTTT29570.1626998602993083No Hit
GAGTACATGGGAAGCAGTGGTATCA28060.1543915481906862No Hit
GTCCTACAGTGTGCATTTCTCATTT18700.10289101750412802No Hit
CTGAAGGACCTGGAATATGGCGAGA18410.10129538140379662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC250.006116315318.9546343
TAGGACC7050.012.6364234
GTCCTAT2000.012.3963451
GGCGAGG10500.012.26656519
GTGTAGG2800.012.0421651
CCGTGCA752.1218215E-411.3721519
CGAAATC1354.8694346E-911.23827813
GACCGTG600.00598177211.0568697
AAGTCGT1900.010.9737357
GTCCTAA11800.010.8415491
TGTACCG803.8555395E-410.6619815
AATGTCC6950.010.6135268
TCCAACG1355.9530066E-810.52278918
AGGACGT24900.010.5049785
CACCTTT5600.010.50232514
GTCATAT1707.403287E-1010.5004341
GTGTAGA4350.010.4870231
TGTCCAC7150.010.47317810
TGGCGAG23050.010.43604918
GGACGTG24650.010.4192816
Produced by FastQC (version 0.11.5)