FastQCFastQC Report
Thu 26 May 2016
SRR1512460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817457
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC74890.41205926742695975No Hit
GTATCAACGCAGAGTACTTTTTTTT61830.3402006209775527No Hit
CCCATGTACTCTGCGTTGATACCAC55580.3058119119186864No Hit
CTGTAGGACGTGGAATATGGCAAGA46490.25579697346347124No Hit
GTCCTACAGTGGACATTTCTAAATT45500.2503498019485468No Hit
GTCCTAAAGTGTGTATTTCTCATTT43700.2404458537395933No Hit
TATCAACGCAGAGTACTTTTTTTTT42790.23543885770062234No Hit
GGTATCAACGCAGAGTACTTTTTTT41770.22982662038221538No Hit
CTTTAGGACGTGAAATATGGCGAGG39600.217886860596977No Hit
GAGTACATGGGAAGCAGTGGTATCA32570.17920644064756414No Hit
CTGAAGGACCTGGAATATGGCGAGA23630.13001683120976176No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21600.118847378507442No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20670.11373033859948269No Hit
GTCCTACAGTGTGCATTTCTCATTT20390.11218972443364547No Hit
CATGTACTCTGCGTTGATACCACTG19800.1089434302984885No Hit
GTATCAACGCAGAGTACATGGGAAG19050.10481678521142453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1700.013.40743418
TCCGCAA500.001500880713.29863212
TAGGACC7000.012.4878664
GGCGAGG11250.011.81775519
CACCTTT6500.011.69014114
ACCTTTT6500.011.39788715
CCAACGT600.00589286611.079144519
AGGACGT24650.011.0630635
GGACGTG24450.010.9176796
TGGCGAG24100.010.83674818
CCTACAC2300.010.7409823
TTAGGAC14650.010.6366453
TGTAGGA25050.010.6205932
ACGAAAT1701.382432E-1010.61655512
CTGTAGG24750.010.5687231
GACGTGG13100.010.5150547
GCCGGTT1553.6634447E-910.41914411
GTAGGAC25350.010.3449783
TAGGACG27450.010.2804614
GACGTGA12850.010.2760567
Produced by FastQC (version 0.11.5)