FastQCFastQC Report
Thu 26 May 2016
SRR1512459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354206
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54820.23285982620042597No Hit
GTCCTACAGTGGACATTTCTAAATT48980.20805316102329194No Hit
GTCCTAAAGTGTGTATTTCTCATTT45670.19399321894515603No Hit
CTGTAGGACGTGGAATATGGCAAGA45380.19276138111957916No Hit
CTTTAGGACGTGAAATATGGCGAGG38310.16273002447534327No Hit
GGTATCAACGCAGAGTACTTTTTTT32510.13809326796380605No Hit
TATCAACGCAGAGTACTTTTTTTTT31830.13520482064866032No Hit
GTCCTACAGTGTGCATTTCTCATTT26950.11447596344584968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8200.012.3661994
CTAGTAC1501.8371793E-1011.372283
GTCCTAC24650.011.0621861
CGTAGAC600.00598461911.0563843
GGCGAGG12700.011.0352319
GTATAGG2250.011.017491
TTAGGAC17900.010.2710533
TCCTACA27900.010.2241832
GGACCTG17450.010.2100716
AGGACGT29100.010.1608015
CTAGGAC3750.010.1086943
TCCAACG750.002723095710.099421518
TAGAAAT8300.010.0477984
CCAGGCG856.744859E-410.0343653
AGGACCT18150.010.025155
TGGCGAG28350.010.01926818
GGACGTG28900.010.0015736
CCTACAG28050.010.0005793
CCTACAC2850.09.9756843
GGTATCA17000.09.974071
Produced by FastQC (version 0.11.5)