FastQCFastQC Report
Thu 26 May 2016
SRR1512459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354206
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69980.29725521046161635No Hit
CTGTAGGACGTGGAATATGGCAAGA52690.22381218975739592No Hit
GTCCTACAGTGGACATTTCTAAATT51750.2198193361158709No Hit
GTCCTAAAGTGTGTATTTCTCATTT48330.2052921452073438No Hit
CTTTAGGACGTGAAATATGGCGAGG46490.19747634658989058No Hit
GGTATCAACGCAGAGTACTTTTTTT46290.19662680326190657No Hit
TATCAACGCAGAGTACTTTTTTTTT45590.1936534016139624No Hit
GTCCTACAGTGTGCATTTCTCATTT27420.11647239026661219No Hit
CTGAAGGACCTGGAATATGGCGAGA25710.10920879481234863No Hit
GTACATGGGAAGCAGTGGTATCAAC25130.1067451191611949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT551.9371824E-413.8379571
TAGGACC9200.012.3940084
ACTGCGT907.475148E-611.6096968
GGCGAGG14050.011.42395519
ATCGTGC700.001493413610.855828
AGGACGT28700.010.8264035
GGTTTAC1002.3622335E-510.4649551
GGACGTG29250.010.45706756
TGTAGGA29600.010.4006962
AGGACCT18100.010.3420345
AGGCGTG1951.4551915E-1110.229747
TGGCGAG29600.010.20353718
GAGGCGT750.00265064810.1323146
ATTTAGA9600.010.1081961
TTTAGAA10750.010.0764022
CGGTAGG856.5094756E-410.0732191
TTAGGAC18500.010.0668463
CTGTAGG30000.09.9892761
GGACCTG18800.09.9537856
GTAGGAC30550.09.9528593