FastQCFastQC Report
Thu 26 May 2016
SRR1512458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137094
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50340.23555351332229654No Hit
GGTATCAACGCAGAGTACTTTTTTT30810.14416773431585134No Hit
GTCCTACAGTGGACATTTCTAAATT30650.14341905409869665No Hit
TATCAACGCAGAGTACTTTTTTTTT30020.14047112574365No Hit
CTGTAGGACGTGGAATATGGCAAGA28500.1333586636806804No Hit
GTCCTAAAGTGTGTATTTCTCATTT26910.1259186540227056No Hit
CTTTAGGACGTGAAATATGGCGAGG22680.1061254207816783No Hit
GTACATGGGAAGCAGTGGTATCAAC22450.10504919296951842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6800.013.3793224
GTATTAG2000.012.8872921
CCGTGCG854.050309E-612.2652449
CGTATCA853.4482247E-511.6627081
GACGTGA9250.010.8601517
GGCGAGG8850.010.8066919
GTGTAGG2950.010.7534121
GTCCTAA9050.010.6253061
TGTAGGA19500.010.5950712
GTTCTAG2250.010.5741891
TGGCGAG18100.010.5140718
GTCCTAC18600.010.4995731
GTTTTAG1801.8189894E-1010.464041
GGACGTG17100.010.3083376
TTAGGAC12000.010.266773
AGGACCT14550.010.2260635
AGGACGT17450.010.15588955
GTAGGAC19850.010.1215523
CTAGACT2250.010.1088214
TCCTACA20400.010.0347282
Produced by FastQC (version 0.11.5)