FastQCFastQC Report
Thu 26 May 2016
SRR1512458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137094
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63050.2950267980725228No Hit
TATCAACGCAGAGTACTTTTTTTTT43710.2045300768239488No Hit
GGTATCAACGCAGAGTACTTTTTTT41030.1919896831866076No Hit
CTGTAGGACGTGGAATATGGCAAGA34060.15937530122680613No Hit
GTCCTACAGTGGACATTTCTAAATT31250.14622660491302675No Hit
GTCCTAAAGTGTGTATTTCTCATTT29130.136306592035727No Hit
CTTTAGGACGTGAAATATGGCGAGG27380.1281179021605975No Hit
GTACATGGGAAGCAGTGGTATCAAC26000.12166053528763826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6750.012.66857054
GGCGAGG7000.011.66826619
TGTAGGA20100.011.39199352
TAGACTG2100.011.3112245
GTAGGAC20650.011.1805943
CTGTAGG19900.011.0895021
GACGTGG10100.010.7218737
AGGACCT13350.010.6757625
CGAGCAC909.50575E-510.5568953
GGACGTG18150.010.3627686
AGGACGT18750.010.2362045
TGGCGAG18700.010.15838918
CCGTCAG750.002650105910.1324859
GGACCTG13000.010.0106996
TCCTACA19300.09.9442672
GTCCTAC17900.09.9372391
ACGTGGA11000.09.9309868
AGGACCG1151.0248839E-59.9145345
CGTGGAA10800.09.7630719
TTAGACT2350.09.7035874
Produced by FastQC (version 0.11.5)