FastQCFastQC Report
Thu 26 May 2016
SRR1512457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558931
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43840.2812183477010849No Hit
GTACATGGGAAGCAGTGGTATCAAC31350.2010993430754793No Hit
GGTATCAACGCAGAGTACTTTTTTT29940.1920546836261515No Hit
TATCAACGCAGAGTACTTTTTTTTT27380.17563317427134365No Hit
CCCATGTACTCTGCGTTGATACCAC21370.13708111520009544No Hit
GTCCTAAAGTGTGTATTTCTCATTT15780.10122321000737043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAC307.8148884E-418.96650713
GGCGCGC400.005449624314.1818678
TCCAACG852.8100294E-713.3674218
GTATTAG1505.456968E-1212.5566541
TAGGACC3400.011.7085634
GACCGTG600.005976218711.0580877
TGTAGGA11900.010.6731112
CTAGCAC1451.4946636E-810.4588793
GTCCTAC9100.010.348891
GTCCTAA5900.010.0811661
GTAGGAC11250.010.0259993
CACTTAG1001.7948751E-49.9131471
GTGTAGG2154.5474735E-119.6826091
TGGCAAG10000.09.65796118
TCCTACA10700.09.6555252
CGTGGAA5700.09.642169
ATGGCAA9950.09.53429217
GTCTAGT1251.6648617E-59.5166221
ACGAAAT900.0011349659.48019412
GACAGCG800.00460116949.478367
Produced by FastQC (version 0.11.5)