FastQCFastQC Report
Thu 26 May 2016
SRR1512457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512457_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558931
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57100.36627663443731634No Hit
TATCAACGCAGAGTACTTTTTTTTT39850.2556238858551148No Hit
GGTATCAACGCAGAGTACTTTTTTT39500.2533787576230122No Hit
GTACATGGGAAGCAGTGGTATCAAC35570.22816917490254537No Hit
CCCATGTACTCTGCGTTGATACCAC26310.16876949653320128No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19010.12194253626363193No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17490.11219226508421476No Hit
CTTTAGGACGTGAAATATGGCGAGG16860.10815103426643001No Hit
CTGTAGGACGTGGAATATGGCAAGA16540.10609834559707901No Hit
GTCCTAAAGTGTGTATTTCTCATTT16520.1059700525552446No Hit
GTCCTACAGTGGACATTTCTAAATT16290.10449468257414858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAT500.00149968413.2998438
AAGGCGT853.946685E-612.2931836
TAGGGTG1406.7848305E-1011.53785
TAGTATC600.00587618911.0832027
GGCGAGG3300.010.93400119
TAGGACT2100.010.4066424
TACTAGC750.002644314710.134842
ACCGTGG750.002647837210.1332148
GTCCTAC10100.010.0791161
TAGGACC3600.09.5017184
ATTAGGC800.00450992549.5014123
TGTAGGA10700.09.5014122
TGGCGAG8900.09.495316518
GTCCTAA5450.09.2520811
AGGACGT9450.09.2503495
GGACGTG9350.09.2452866
TAGGCTG1856.670234E-99.2449135
GTTCCTA1651.0410622E-79.2256311
CCTATAC1252.7481821E-59.1213553
GACGTGA4900.09.1121377