FastQCFastQC Report
Thu 26 May 2016
SRR1512456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308386
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40410.3088538091969801No Hit
GGTATCAACGCAGAGTACTTTTTTT25430.19436160276860193No Hit
GTCCTACAGTGGACATTTCTAAATT24650.1884000593097144No Hit
TATCAACGCAGAGTACTTTTTTTTT24360.18618358802371776No Hit
CTGTAGGACGTGGAATATGGCAAGA22860.17471908137201103No Hit
GTCCTAAAGTGTGTATTTCTCATTT22240.16998041862263888No Hit
CTTTAGGACGTGAAATATGGCGAGG19390.14819785598439603No Hit
GTACATGGGAAGCAGTGGTATCAAC18800.14368848336805806No Hit
GTCCTACAGTGTGCATTTCTCATTT14370.10982997372335077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4550.013.1237734
GTCCTAC12550.011.6973871
TATGTCG658.1327057E-411.67319816
TGTCGAG658.251445E-411.65304418
ACTGATC1157.561357E-811.5091588
TGTAGGA15550.011.3983182
GTGTAGG2350.011.3963761
GCCTATA700.001039546411.3360791
ATAGGAC1354.8949005E-911.2335183
GTCGAGG600.00602543711.04569119
GTAGGAC15300.010.903123
GTCCTAT1551.7935236E-910.8789791
TCCTACA14500.010.7856292
CTGTAGG14450.010.5711871
CCTACAG14050.010.5239273
GGCGAGG7250.010.44715619
AGGACGT16150.010.387965
GGACGTG15950.010.3399426
GTCTTAA1251.4771831E-610.3158311
GTAGGAG1303.9602673E-710.2073783
Produced by FastQC (version 0.11.5)