FastQCFastQC Report
Thu 26 May 2016
SRR1512456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308386
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54290.41493871074744No Hit
GGTATCAACGCAGAGTACTTTTTTT36930.28225615376502045No Hit
TATCAACGCAGAGTACTTTTTTTTT34210.26146718170325883No Hit
CTGTAGGACGTGGAATATGGCAAGA27870.2130105335887116No Hit
GTCCTACAGTGGACATTTCTAAATT27610.2110233524357491No Hit
GTCCTAAAGTGTGTATTTCTCATTT25520.19504947316770432No Hit
CTTTAGGACGTGAAATATGGCGAGG23180.17716484279104178No Hit
GTACATGGGAAGCAGTGGTATCAAC20860.15943307250306868No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17820.1361983390222763No Hit
CCCATGTACTCTGCGTTGATACCAC15480.11831370864561375No Hit
GTCCTACAGTGTGCATTTCTCATTT15030.11487435665010173No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14200.10853066296949065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG853.9562638E-612.29013619
TACACCG658.007722E-411.6947795
CTAGACA907.4403943E-611.6135654
GGCGAGG6350.011.21683919
CTAACAC1201.2705823E-711.0856753
TAGGACC4200.010.63324
TTAGTAC909.4911506E-510.5577873
TATACTG2100.010.4069615
TAATACC1303.8267535E-710.2329324
AGGACGT16350.010.228465
GAAATGT3900.010.2282386
GGACGTG15700.010.2235856
TCCTAGG1502.433626E-810.1354752
GTCATAA951.6159793E-410.0166681
GATATAC1909.640644E-1110.0166681
TATACAC4850.09.9918023
GTCCTAA8500.09.9636381
GGTCTAC1151.0056332E-59.9295671
TAAGACC1252.5609115E-69.8820884
CAAGTCG1252.5759746E-69.8775556
Produced by FastQC (version 0.11.5)