FastQCFastQC Report
Thu 26 May 2016
SRR1512455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130715
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46350.40991761849803No Hit
GGTATCAACGCAGAGTACTTTTTTT28890.2555020495880925No Hit
TATCAACGCAGAGTACTTTTTTTTT27660.24462397686419654No Hit
GTACATGGGAAGCAGTGGTATCAAC25300.22375222757281898No Hit
CCCATGTACTCTGCGTTGATACCAC18060.15972194584842334No Hit
GTCCTACAGTGGACATTTCTAAATT17000.15034734659043172No Hit
GTCCTAAAGTGTGTATTTCTCATTT15110.13363225923420136No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14040.12416922036056831No Hit
CTGTAGGACGTGGAATATGGCAAGA13900.1229310657415883No Hit
CTTTAGGACGTGAAATATGGCGAGG13080.115679017258991No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12530.11081483839871231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGC250.00613387718.94447119
ACTATAC1201.0359145E-811.846613
GTCCTAC9700.011.32904151
TACTCCG600.005979526311.0568365
CTACACT1803.6379788E-1211.0568364
TAGGACC3100.011.0058824
GAATAAC906.253538E-511.000171
GTGTAAG1654.5292836E-1010.8001681
TTAGGAC6700.010.7503573
GATTAGG750.001878334110.56016351
TAGGCTG1355.8809746E-810.530325
CCTACAC1553.801688E-910.3944443
TAGGACG10800.010.3548154
TCCTACA11350.010.3540412
ATTGAAC1106.2142044E-610.338863
TGTAGGA10200.010.3135192
GTTCTAA1251.5130936E-610.2961591
AGGACGT10450.010.2481685
GGACGTG10450.010.2481686
GTAGGAC9250.010.2457163
Produced by FastQC (version 0.11.5)