FastQCFastQC Report
Thu 26 May 2016
SRR1512455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130715
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54060.47810456215757285No Hit
TATCAACGCAGAGTACTTTTTTTTT37690.33332891135255127No Hit
GGTATCAACGCAGAGTACTTTTTTT37000.32722657787329257No Hit
GTACATGGGAAGCAGTGGTATCAAC28700.2538216968909053No Hit
CCCATGTACTCTGCGTTGATACCAC21780.19262148286703545No Hit
GTCCTACAGTGGACATTTCTAAATT18590.16440924547741917No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17780.1572456366104633No Hit
CTGTAGGACGTGGAATATGGCAAGA17760.15706875737918044No Hit
GTCCTAAAGTGTGTATTTCTCATTT16580.14663288273349162No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16250.14371437541732443No Hit
CTTTAGGACGTGAAATATGGCGAGG15580.1377889211693486No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12450.11010732147358088No Hit
GAGTACATGGGAAGCAGTGGTATCA12070.10674661607920652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTC456.7554193E-414.7791899
GTTTTAG852.6648013E-713.4249221
AGGGTCG604.0887247E-412.6673165
TAAGACG550.003067395612.0909944
TAGGACC2900.011.7931864
CCTAGGC1052.7184433E-711.7614663
CCTAATA855.32167E-511.1760552
GGCGAGG3700.011.03768819
GTAGGAC10500.011.03768353
TGTAGGA11300.011.0128632
GTCTATA1053.4224304E-610.8677931
GACGTGG5400.010.7325067
CTGTAGG10800.010.653961
ACGTGGA5500.010.3646268
AGGACGT10000.010.355535
GGACGTG10200.010.3387656
GAACAGT1303.8331746E-710.2312936
CGTGGAA5450.010.1110579
GTCATAC856.5308326E-410.0686911
TATACTG1701.553417E-910.0593395
Produced by FastQC (version 0.11.5)