FastQCFastQC Report
Thu 26 May 2016
SRR1512454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104244
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47700.22668473808170533No Hit
GTACATGGGAAGCAGTGGTATCAAC46390.2204592243104887No Hit
CCCATGTACTCTGCGTTGATACCAC33400.15872683966308088No Hit
GGTATCAACGCAGAGTACTTTTTTT30510.14499269096169456No Hit
TATCAACGCAGAGTACTTTTTTTTT29320.1393374532611237No Hit
CTGTAGGACGTGGAATATGGCAAGA24290.11543338129988727No Hit
GTCCTACAGTGGACATTTCTAAATT23080.10968309758754213No Hit
GTCCTAAAGTGTGTATTTCTCATTT22360.10626144116366733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCG400.005373648414.2122489
TACCTCG500.001527654913.2676155
TAGACTC1800.012.6358245
TACGCTG604.178115E-412.6358245
GGATCGT658.19126E-411.6638376
GACGTGA8700.011.5465297
GGACGTG14900.011.44856556
GTACCGT1001.9878808E-611.3722426
GTCCTAC10950.011.3190191
AGGACGT15500.011.1888195
GTCTTAG2350.010.9702971
TTAGACT1303.38714E-810.9348484
GTCCTAA7750.010.8750211
TTAGGAC10550.010.86920453
TGCGCCA1053.5620706E-610.83277712
GGCGAGG7600.010.71819319
GCACCGT803.857772E-410.6614776
ACGTGAA9850.010.6543248
CTTAGAC1252.3175016E-710.6140933
TAGGACG17100.010.5852744
Produced by FastQC (version 0.11.5)