FastQCFastQC Report
Thu 26 May 2016
SRR1512454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104244
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62900.2989197070301733No Hit
GTACATGGGAAGCAGTGGTATCAAC53760.25548367964931823No Hit
TATCAACGCAGAGTACTTTTTTTTT42720.20301828114990464No Hit
GGTATCAACGCAGAGTACTTTTTTT41860.19893130264360975No Hit
CCCATGTACTCTGCGTTGATACCAC40750.19365624899013612No Hit
CTGTAGGACGTGGAATATGGCAAGA28310.13453762966652158No Hit
GTCCTACAGTGGACATTTCTAAATT24550.11666897945295318No Hit
GTCCTAAAGTGTGTATTTCTCATTT24120.11462549019980572No Hit
GAGTACATGGGAAGCAGTGGTATCA22650.10763960833439469No Hit
CTTTAGGACGTGAAATATGGCGAGG21200.10074877248075792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4100.012.5157514
GTCCTAA8050.010.40140151
ATTTAGA5050.010.36278251
TCTATAC1752.3464963E-1010.3169713
GTCCTAC13850.010.2362661
ATAAGAC1409.655014E-810.1812223
GTATAAT2900.010.171121
TACACCG856.5763685E-410.061925
GGCGAGG6900.010.04641319
CTTATAC2300.09.9286111
TCCTACA15050.09.8495062
GTGTTAT1451.5809565E-79.84301951
TTTAGAA5900.09.8243582
GTCTTAC1951.5825208E-109.7588911
AGGACGT13800.09.7095115
GGACGTG13450.09.6758786
TGTAGGA15100.09.5651772
AGGACCT9450.09.55320555
CCTACAG15350.09.5334263
GTCTAGA2106.548362E-119.5149191
Produced by FastQC (version 0.11.5)