FastQCFastQC Report
Thu 26 May 2016
SRR1512453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257656
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45440.361307066479228No Hit
GGTATCAACGCAGAGTACTTTTTTT30700.24410490627007703No Hit
TATCAACGCAGAGTACTTTTTTTTT27600.2194558766467142No Hit
GTACATGGGAAGCAGTGGTATCAAC23430.18629895615335196No Hit
CCCATGTACTCTGCGTTGATACCAC17910.14240778082400912No Hit
GTCCTAAAGTGTGTATTTCTCATTT15560.12372222610952438No Hit
CTGTAGGACGTGGAATATGGCAAGA14770.11744069920550612No Hit
GTCCTACAGTGGACATTTCTAAATT14690.11680459521522578No Hit
CTTTAGGACGTGAAATATGGCGAGG13560.10781962635251612No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13390.1064679053731704No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13280.10559326238653495No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG402.8094917E-416.5867635
CCGTGCG508.89666E-515.1626219
GTCTACG400.0039977114.8588281
GGTTGAG905.5550845E-712.6375347
CTACACT1451.0186341E-1011.7659814
GTGTAGA1501.0659278E-911.226671
GTTGTAA1551.8335413E-910.8645191
CTAAGGT1159.074538E-710.7144324
GGTCTAT650.007803269710.6678761
ACTACAC1252.3099528E-710.6155293
TAGGACC2950.010.6026764
CTTACAC1355.8756996E-810.5312783
ACGAAAT1002.4547135E-510.4276312
GTTCTGG2200.010.3561531
GTCCTAT1156.231703E-610.3365761
GGCGAGG4600.010.29617419
ATGTCCA3500.010.288929
GTTCTAG1552.1209416E-810.225431
GTCCTAA6600.010.2060631
TGTCCAC4100.010.17248510
Produced by FastQC (version 0.11.5)