FastQCFastQC Report
Thu 26 May 2016
SRR1512453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257656
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58990.46904717983295907No Hit
GGTATCAACGCAGAGTACTTTTTTT44270.3520040456213782No Hit
TATCAACGCAGAGTACTTTTTTTTT41590.3306945619469871No Hit
GTACATGGGAAGCAGTGGTATCAAC27160.21595730470017238No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19270.15322154865877471No Hit
CTGTAGGACGTGGAATATGGCAAGA17400.138352617885972No Hit
CCCATGTACTCTGCGTTGATACCAC17120.13612625391999084No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16530.13143498699167339No Hit
CTTTAGGACGTGAAATATGGCGAGG15650.12443784309858975No Hit
GTCCTAAAGTGTGTATTTCTCATTT15240.12117781014840305No Hit
GTCCTACAGTGGACATTTCTAAATT14200.11290845827475876No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13510.10742206135859089No Hit
GAGTACATGGGAAGCAGTGGTATCA12680.10082248245943247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG651.915032E-716.0750067
AACGTCT400.005282539414.2483017
TAATACC500.001497473313.3021164
TCGAATT500.001501067513.29788514
TAGGACC3350.012.4795964
TACACCG658.0108555E-411.6941685
TTATACC855.3121392E-511.1782484
TTTAGAC600.005867648411.0850963
GTTGTAA1002.3628789E-510.463731
ACAGTCC1752.3646862E-1010.3130568
TAGGCTG1201.519249E-610.29330355
AGAACGG750.00264348110.1349455
TGGCGAG9900.010.07415518
GCATTAA856.5119826E-410.072041
GTTATAC856.5814465E-410.0604243
TAGAAAT3500.010.0444554
GCCTTAC951.6216245E-410.0131391
GAGTAGG1252.5248064E-69.8929821
ATTACAC1356.4215965E-79.8534183
TTAGGAC6700.09.7851383
Produced by FastQC (version 0.11.5)