FastQCFastQC Report
Thu 26 May 2016
SRR1512452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894512
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37590.4202291305203284No Hit
GGTATCAACGCAGAGTACTTTTTTT23630.26416638345824317No Hit
TATCAACGCAGAGTACTTTTTTTTT22380.25019228361385876No Hit
GTACATGGGAAGCAGTGGTATCAAC18890.2111765968483374No Hit
GTCCTACAGTGGACATTTCTAAATT15900.17775055002056986No Hit
GTCCTAAAGTGTGTATTTCTCATTT14470.1617641797985941No Hit
CTGTAGGACGTGGAATATGGCAAGA13970.15617453986084032No Hit
CTTTAGGACGTGAAATATGGCGAGG13100.14644856636914877No Hit
CCCATGTACTCTGCGTTGATACCAC12980.14510705278408786No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12380.13839948485878334No Hit
GTCCTACAGTGTGCATTTCTCATTT11600.12967964655588746No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11110.12420179941688876No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9050.10117248287334323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3400.014.4976244
ATCCTAC551.3624316E-414.4214511
GTGTAGG1401.8189894E-1213.4557291
TTGCACC604.165216E-412.6389544
GTCGAGG604.2088368E-412.62335319
CTTGCAC854.03557E-612.26722053
ATAAGAC951.0659278E-611.9737463
TAAGACA1353.8380676E-1011.9367914
TGTCGAG658.25509E-411.65167418
TAGAGTG752.1144899E-411.3750595
TTATGCT752.1144899E-411.3750594
GGCGAGG4350.011.09984719
ATTATAC1201.3114004E-711.0590853
TAGAACC700.001517794110.833394
GTCCTAG1208.777406E-710.7409761
TATGTCG803.8124633E-410.67371216
TCAGGAC1605.638867E-1010.6641183
GAAATGT2150.010.581456
GTGTTAC750.001854753910.575731
TCCTACT909.717037E-510.5324622
Produced by FastQC (version 0.11.5)