FastQCFastQC Report
Thu 26 May 2016
SRR1512452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894512
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50150.560640885756703No Hit
TATCAACGCAGAGTACTTTTTTTTT35940.4017833187257409No Hit
GGTATCAACGCAGAGTACTTTTTTT34840.38948611086268264No Hit
GTACATGGGAAGCAGTGGTATCAAC21890.24471443647486002No Hit
CTGTAGGACGTGGAATATGGCAAGA16690.18658218112222083No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16080.17976282039816124No Hit
GTCCTACAGTGGACATTTCTAAATT15690.1754029012467133No Hit
CTTTAGGACGTGAAATATGGCGAGG15410.17227270288157118No Hit
GTCCTAAAGTGTGTATTTCTCATTT14900.16657127014506234No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14580.16299390058489993No Hit
CCCATGTACTCTGCGTTGATACCAC14170.15841039583594185No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11990.13403956570733538No Hit
GTCCTACAGTGTGCATTTCTCATTT10500.11738243869282916No Hit
GAGTACATGGGAAGCAGTGGTATCA10130.11324610513889137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3900.012.1814954
GACTGCG550.003066761712.0908737
TGTAGGA9700.012.0477042
GTAGGAC9800.012.0217183
ATAGGAC1052.710858E-711.7631863
TCTTATA1052.710858E-711.7631862
TCGTCAA1052.714787E-711.76187110
CTGTAGG10050.011.6463731
CAAGTCG1001.9277377E-611.3993296
AAGTCGT1104.958747E-711.227247
AGGACCT6950.011.0737685
GGACCTG6550.011.0222526
AGGACGT9000.010.97958855
GGACGTG8950.010.7200396
GTCTAGA803.7093804E-410.705431
GGATAGA803.7093804E-410.705431
GTCCTAA5100.010.6354611
GACGTGG4650.010.6236257
GTATAAA909.3435956E-510.5732651
ATGGCAA8450.010.56447117