FastQCFastQC Report
Thu 26 May 2016
SRR1512451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551075
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53540.2098723087325931No Hit
GTACATGGGAAGCAGTGGTATCAAC52050.20403163372303834No Hit
CCCATGTACTCTGCGTTGATACCAC38720.1517791519261488No Hit
GGTATCAACGCAGAGTACTTTTTTT32120.12590770557510067No Hit
TATCAACGCAGAGTACTTTTTTTTT30960.1213606028830983No Hit
GTCCTACAGTGGACATTTCTAAATT28690.11246239330478328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGA701.11416164E-412.18733410
GTACTAG1706.548362E-1111.0680471
GTCCTAG3450.010.907641
TAGGACC6000.010.9008934
TATAGCG700.001519710310.8331865
GTCCTAA8900.010.5706061
CTAGGAC3450.010.4406793
GTCTTAA2900.010.2444721
GGCGAGG8650.010.07262719
GTCTAGA3650.010.0386471
TAGACTG2850.09.9779355
GGTCTAA1501.4474426E-79.9029891
TAGGACA6550.09.8408334
TGGCGAG16950.09.83247318
GTCCTAC14350.09.7994741
TTATACT3500.09.7498674
CCTAGAC1951.70985E-109.722093
AGGACGT16750.09.6771075
TTAGGAC11500.09.643893
GTGTAGG4050.09.5362111
Produced by FastQC (version 0.11.5)