FastQCFastQC Report
Thu 26 May 2016
SRR1512451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551075
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66040.2588712601550327No Hit
GTACATGGGAAGCAGTGGTATCAAC60840.23848769636329784No Hit
TATCAACGCAGAGTACTTTTTTTTT45210.1772194075046794No Hit
GGTATCAACGCAGAGTACTTTTTTT44520.17451466538616073No Hit
CCCATGTACTCTGCGTTGATACCAC43480.17043795262781375No Hit
GTCCTACAGTGGACATTTCTAAATT29640.11618631361288867No Hit
CTGTAGGACGTGGAATATGGCAAGA29260.11469674548964652No Hit
GAGTACATGGGAAGCAGTGGTATCA27540.10795448977391883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCG456.763216E-414.7781284
TAGGACC6100.012.7707964
AAGTCGT1701.0913936E-1111.1765177
TATACCG700.001491675310.85745
AACCGTG803.7723168E-410.6875457
TGGCGAG15050.010.66572818
CTAGTAC1451.4440957E-810.4830073
CTACACG1451.4440957E-810.4830074
TAGGACA5650.010.4250274
GTAGGAC16150.010.1767113
GGCGAGG7000.010.1760219
CAAGTCG1606.177288E-910.0937926
GGACGTG15750.09.7111516
TGTAGGA18300.09.7078822
TTAAGAC2650.09.6794753
CTAGGAC3250.09.6463823
TTAGGAC9850.09.6448983
AGGACGT15600.09.6220245
TATGGCG16150.09.52758316
CTAAGAC2600.09.5002263
Produced by FastQC (version 0.11.5)