FastQCFastQC Report
Thu 26 May 2016
SRR1512450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822020
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48380.26552946729454124No Hit
GTCCTACAGTGGACATTTCTAAATT41070.22540916126057892No Hit
GTCCTAAAGTGTGTATTTCTCATTT39730.21805468655667887No Hit
CTGTAGGACGTGGAATATGGCAAGA38500.21130393738817357No Hit
CTTTAGGACGTGAAATATGGCGAGG32100.17617808805611354No Hit
GGTATCAACGCAGAGTACTTTTTTT29410.16141425450873206No Hit
GTACATGGGAAGCAGTGGTATCAAC28590.15691375506306188No Hit
TATCAACGCAGAGTACTTTTTTTTT27240.14950439621958048No Hit
CCCATGTACTCTGCGTTGATACCAC25210.13836291588456767No Hit
GTCCTACAGTGTGCATTTCTCATTT23550.1292521487140646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT551.9985432E-413.7868536
CGTCCGG500.001525585113.26984610
TAGGACC7750.012.1079714
AATGTCC6700.011.1561258
GCAAACG600.005953171811.06399513
GTTCTAA1801.6370905E-1110.9829451
GTCCTAA13150.010.7490881
GGCGAGG9550.010.51972819
CTACACT3900.010.4506124
TGTCCAC7500.010.36311810
GAAATGT7700.010.340146
AGAACAG5450.010.2610875
TCCAACG1856.184564E-1110.2450118
ATGTCCA7250.010.19527159
TGTAGGA24250.010.1233892
AGGACCG750.002698259410.1103595
TCCTAGA3750.010.1103592
GACAGCG856.733099E-410.0360187
CCACCTT6450.09.9980313
TAGGACT3800.09.9773294
Produced by FastQC (version 0.11.5)