FastQCFastQC Report
Thu 26 May 2016
SRR1512450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512450_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822020
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61230.336055586656568No Hit
TATCAACGCAGAGTACTTTTTTTTT43930.24110602518084323No Hit
CTGTAGGACGTGGAATATGGCAAGA42850.2351785381060581No Hit
GGTATCAACGCAGAGTACTTTTTTT41990.23045850210206253No Hit
GTCCTACAGTGGACATTTCTAAATT37730.20707785864041006No Hit
GTACATGGGAAGCAGTGGTATCAAC37280.20460807235924963No Hit
GTCCTAAAGTGTGTATTTCTCATTT35720.19604614658456002No Hit
CTTTAGGACGTGAAATATGGCGAGG35640.19560707346790926No Hit
CTGAAGGACCTGGAATATGGCGAGA21310.1169581014478436No Hit
GTCCTACAGTGTGCATTTCTCATTT21070.11564088209789136No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20860.11448831516668315No Hit
CCCATGTACTCTGCGTTGATACCAC20500.11251248614175474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8150.011.0756674
GGCGAGG11250.010.80614119
GTTAGAC803.764906E-410.6894773
CAGCGTT803.7744688E-410.6865439
GGACGTT803.7744688E-410.6865436
AAGGCGT803.7744688E-410.6865436
TTAGGAC14600.010.4779643
AATGTCC6850.010.4005288
AGGACGT22300.010.3116075
TAGGACT3050.010.280594
TGGCGAG24800.010.110332518
AGGACCT15800.010.1034125
TAAGACC2450.010.0834974
GGACGTG21900.010.0630266
ACCGTGC856.5995345E-410.0579238
AAATGTC7000.010.0419587
GTCCTAA12800.010.0326781
TGTCCAC7350.09.95148910
TCGAACT1054.116747E-59.94985119
TAGAAAT7550.09.9422364